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The primer sequences used for real-time quantitative PCR.
| Gene | Primer sequences (5′—3′) | Length (bp) |
|---|---|---|
| GAPDH | Forward: GAACGGGAAGCTCACTGG | 123 |
| Reverse: GCCTGCTTCACCACCTTCT | ||
| Bax | Forward:AGCTGCAGAGGATGATTGCTG | 178 |
| Reverse:CTGATCAGCTCGGGCACTTTA | ||
| Bcl-2 | Forward:TGGCCTTCTTTGAGTTCGGT | 111 |
| Reverse:GATGCCGGTTCAGGTACTCA | ||
| Cytochrome c | Forward:CTTGGGCTAGAGAGCGGGA | 179 |
| Reverse:CCATGGAGGTTTGGTCCAGT | ||
| Caspase-9 | Forward:AGCTGGCCCAGTGTGAATAC | 243 |
| Reverse:GCTCCCACCTCAGTCAACTC |
