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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Nature. 2022 May 11;605(7910):509–515. doi: 10.1038/s41586-022-04722-0

Figure 3. SRF signaling is downregulated in hippocampal OPCs with ageing and induced by acute young CSF injection.

Figure 3.

a, Srf mRNA quantified in OPC (Pdgfrα+ nuclei) in the CA1 region of the hippocampus of young (3 months) and aged (22 months) mice. (young n=6, aged n=7; two-sided t-test; mean ± s.e.m.).

b, Representative images of a. Scale bar, 10μm and 5μm in insert.

c, Volcano plot of DEGs of aged vs. young hippocampal OPC nuclei. Dashed line represents p.adj=0.05 (n=4).

d, Pathways enriched (red) or depleted (blue) in hippocampal OPCs with age. Resource categories; # CellMarker; ## Wikipathways; ### GO BP (n=4, unweighted Kolmogorov-Smirnow test).

e, Box plot of effect size of Srf targets (TRANSFAC database) in hippocampal OPCs from aged vs. young, YM-CSF vs. aCSF at 1hr and 6hr timepoints (n=4; genes pre-filtered by p<0.05 cutoff; Wilcoxon rank sum test; box show the median and the 25–75th percentiles, and the whiskers indicate values up to 1.5-times the interquartile range).

f, Pathways enriched (red) or depleted (blue) in hippocampal OPCs following 6 hrs of aCSF or YM-CSF injection (n=4). Resource categories; # KEGG; ## GP MF (unweighted Kolmogorov-Smirnow test).

g, Meta-analysis of log2FC of SRF target genes (TRANSFAC) in human AD vs. control and mouse aged vs. young ageing datasets (genes pre-filtered by p<0.05 cutoff; box show the median and the 25–75th percentiles, and the whiskers indicate values up to 1.5-times the interquartile range).