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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Nature. 2022 May 11;605(7910):509–515. doi: 10.1038/s41586-022-04722-0

Extended data Fig. 3. Young CSF increases number of myelinated axons in the molecular layer.

Extended data Fig. 3.

a, Representative overview of 1mm diameter biopsy punch in the hippocampus.

b, Representative overview of molecular layer (MoL, between dashed lines) before and after TEM imaging of three 10×10 montage squares (n=7).

c, Representative montage of MoL of aged mouse infused with aCSF and YM-CSF (n=7). Scale bar, 10 μm.

d, Representative higher resolution image of aged mouse infused with aCSF and YM-CSF (n=7). Scale bar, 1μm.

e, g-ratio analysis of myelinated axons in molecular layer. (n=3 mice per group, aCSF n=321 axons, YM-CSF n=291 axons).