FIG. 4.

Time course of the hydrolyzed compounds released from peptidoglycan with the H-LytF enzyme. The time course of the absorbance of peptidoglycan on H-LytF digestion is also shown. After 0-, 10-, 20-, and 60-min digestions of peptidoglycan, the reaction mixtures were centrifuged, and the amino groups of the released peptides and/or mucopeptides in the supernatants were labeled with 1-fluoro-2,4-dinitrobenzene. The labeled compounds were hydrolyzed with a high concentration of HCl, followed by separation by reverse-phase HPLC. Only the amounts of DNP derivatives of diaminopimelic acid were considerably increased during incubation. ■, cell wall turbidity; ▵, mono-dinitrophenyl diaminopimelic acid; ○, bis-dinitrophenyl diaminopimelic acid.