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. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: Nat Genet. 2020 Oct 19;52(12):1283–1293. doi: 10.1038/s41588-020-00731-9

Fig. 2. Evaluation of ACE2 and dACE2 promoters.

Fig. 2.

a, Promoters of ACE2 (P1 and P2) and dACE2 (P3) were analyzed for binding motifs of transcription factors relevant for IFN signaling. Promoters were defined within the −800/+100 bp windows from the corresponding transcription start sites (TSS). b, Schematics of Luciferase (Luc) reporter constructs. c, Luciferase activity in HepG2 cells transiently co-transfected with indicated Luciferase reporter constructs and Renilla (normalization control) and treated with 1 ng/ml of IFNβ or 2 ng/ml of IFNγ for 6 hrs. d, Luciferase activity driven by the promoter of IFIT1 (an ISG and positive control). Luciferase/Renilla ratios were normalized by corresponding mock-treated samples and presented as fold change to the negative control (empty promoterless pGL4.21 vector). P-values are for unpaired, two-sided Student’s T-tests. The experiment was conducted in 6 biological replicates per construct, and the results of one of three independent experiments are shown.