Figure 2. P4HA3 knockdown inhibited adipocyte differentiation in 3T3-L1 cells. A, Expression of P4HA3 following siRNA treatment was detected by qRT-PCR. B and C, C/EBP-α and PPAR-γ expression during the course of adipocytes differentiation was quantified using RT-qPCR and western blot assay after P4HA3 knockdown. D, Representative Oil Red O staining of differentiated adipocytes with or without P4HA3 knockdown (scale bar, 20 μm). E and F, Triglycerides (TG) and glucose uptake determination in differentiated adipocytes with or without P4HA3 knockdown. NC: negative control. Data are reported as means±SD. **P<0.01; ***P<0.001 (ANOVA and t-test).
