Figure 4. P4HA3 silencing modulated adipocyte differentiation and insulin sensitivity gene expressions in adipose tissue. A, Epididymal and inguinal fat pad weight (g) of db/db mice were measured. B, The cross-sectional area of adipocytes in adipose tissue was analyzed by HE staining (scale bar, 200 μm). C and D, C/EBP-α, PPAR-γ, and inflammatory factors (MCP1, interleukin (IL)-6, and IL-1) expression in epididymal and inguinal adipose tissues of db/db mice with or without P4HA3 knockdown was quantified using RT-qPCR. Mice were sacrificed after 6-h fasting for tissue collection. E, The phosphorylation levels of FoxO1, IRS, PI3K, and AKT in epididymal and inguinal adipose tissues of db/db mice were analyzed by western blotting. HFD: high fat diet; NC: negative control. Data are reported as means±SD. **P<0.01; ***P<0.001 (t-test).