Figure 5. P4HA3 improves hepatic glucose homeostasis and steatosis in db/db mice. A, The expression levels of gluconeogenesis genes (G6PC and PCK1) and glycogenolytic gene (PYGL) in liver tissues of db/db mice were determined by RT-qPCR. Mice were sacrificed after 6-h fasting for tissue collection. B, The phosphorylation levels of FoxO1, IRS, PI3K, and AKT in liver tissues of db/db mice with or without P4HA3 knockdown were analyzed using western blotting. C, Hepatic steatosis and liver triglycerides (TG) levels in db/db mice with or without P4HA3 knockdown were examined by HE staining (scale bar, 20 μm) and TG kit. HFD: high fat diet; NC: negative control. Data are reported as means±SD. **P<0.01, ***P<0.001 (t-test).
