FIG. 5.

Cell length and number of nucleoids for ponA mutant (A) and wild-type (B) cells grown at 30°C in 2× YT medium. Images similar to those shown in Fig. 4 were used for the analysis. Cell lengths were measured as described in Materials and Methods, and the number of visible, fully segregated nucleoids present in each cell was counted. A total of 98 and 137 cells are depicted in panels A and B, respectively. The mean cell length and nucleoid content obtained were, respectively, 6.4 μm and 2.5 for ponA mutant cells and 3.9 μm and 1.8 for wild-type cells.