TABLE 1.

PCR primers used in this study

Primer	Nucleotide sequencea
ponAP1	5′-CCGGAATTCCGGCAGCTACTCCG-3′
ponAP2	5′-CGCGGATCCGACCCAATACC-3′
ponAP3	5′-CCATTGAAAAAACAAATGACTACAAGGACCACGATGACAAGTAAACAAAAAAGCCG-3′
ponAP4	5′-GGGAATGGGAGAAAAC-3′
pFLAG	5′-GACTACAAGGACGACGATGACAAG-3′
p1219	5′-ATGCGTCCGGCGTAGA-3′

^aRestriction endonuclease sites are underlined; nucleotides encoding the FLAG epitope are shown in bold.