Skip to main content
. 2022 Mar 4;21(3):460–470. doi: 10.1158/1535-7163.MCT-21-0240

Figure 4.

Figure 4. The oncolytic effect in vitro is vector-type– and cell line–dependent. Comparison of oncolytic effect in vitro of selectively replicating Ad based on Ad 1, 2, and 6 carrying the 24-bp deletion and/or encoding P19. As controls, respective WT viruses (Ad1, Ad2, Ad6) and an Ad5-based vector carrying the 24-bp deletion were explored. Uninfected cells were used as negative control. To perform an oncolysis assay, PANC-I cell (A) and A549 cells (B) were infected at different viral particle numbers per cell (VP/cell) and stained after 4 to 7 days. Representative slides are displayed. Oncolytic effect was quantified by measuring intensity and is displayed as cell viability 4 to 7 days after infection. In PANC-I cells, modified Ad2 viruses showed most potent oncolytic activity and the Ad2 OAd containing P19 and 24-bp deletion revealed highest oncolytic potency. In A549 cells, oncolysis potency was cell line– and Ad-type–dependent. One biological replicate is shown.

The oncolytic effect in vitro is vector-type– and cell line–dependent. Comparison of oncolytic effect in vitro of selectively replicating Ad based on Ad 1, 2, and 6 carrying the 24-bp deletion and/or encoding P19. As controls, respective WT viruses (Ad1, Ad2, Ad6) and an Ad5-based vector carrying the 24-bp deletion were explored. Uninfected cells were used as negative control. To perform an oncolysis assay, PANC-I cell (A) and A549 cells (B) were infected at different viral particle numbers per cell (VP/cell) and stained after 4 to 7 days. Representative slides are displayed. Oncolytic effect was quantified by measuring intensity and is displayed as cell viability 4 to 7 days after infection. In PANC-I cells, modified Ad2 viruses showed most potent oncolytic activity and the Ad2 OAd containing P19 and 24-bp deletion revealed highest oncolytic potency. In A549 cells, oncolysis potency was cell line– and Ad-type–dependent. One biological replicate is shown.