| Gene |
ACTN2 (human) |
https://www.ncbi.nlm.nih.gov/gene/88
|
HGNC:HGNC:164 |
|
| Cell line |
NCRM-1 (Homo sapiens) |
RUCDR, Rutgers University; Chal et al., 2016; Al Tanoury et al., 2021
|
|
|
| Cell line |
GFP-titin (Homo sapiens) |
Derived from PGP-1; Sharma et al., 2018
|
|
|
| Cell line |
mKate2-α-actinin2 |
Derived from NCRM-1, this study |
|
|
| Antibody |
Rabbit-anti-titin (N-term) |
Sigma, Cat. HPA007042 |
|
1 in 50 |
| Antibody |
Mouse anti-MyHC |
DSHB, Clone A4.1025 |
|
5 ng/µL |
| Antibody |
Rat anti-β1integrin |
DSHB, Clone AIIB2 |
|
5 ng/µL |
| Antibody |
Mouse anti-paxillin |
Invitrogen, Cat. AHO0492 |
|
1 in 5 |
| Antibody |
Anti-NCAM1 |
DSHB, Clone 5-1H11 |
|
5 ng/µL |
| Antibody |
Goat anti-mouse Alexa405 |
Invitrogen, Cat. A31553 |
|
1 in 250 |
| Antibody |
Goat anti-rabbit Alexa405 |
Invitrogen, Cat. A31556 |
|
1 in 250 |
| Antibody |
Goat anti-rabbit Alexa488 |
Life Technologies, Cat. A11034 |
|
1 in 250 |
| Antibody |
Goat anti-rat Alexa488 |
Invitrogen, Cat. A11006 |
|
1 in 250 |
| Antibody |
Goat anti-mouse Alexa488 |
Invitrogen, Cat. A11001 |
|
1 in 250 |
| Antibody |
Goat anti-rat Alexa633 |
Life Technologies, Cat. A21094 |
|
1 in 250 |
| Antibody |
Goat anti-rabbit Alexa633 |
Invitrogen, Cat. A21070 |
|
1 in 250 |
| Sequence-based reagent |
mKate2 dsDNA |
Synthesised and obtained from Integrated DNA Technologies |
|
|
| Commercial assay or kit |
Culture-insert 4 well in µ-dish 35 mm |
ibidi, Cat. 80466 |
|
|
| Commercial assay or kit |
Micro-insert 4 well in µ-dish 35 mm |
ibidi, Cat. 80406 |
|
|
| Chemical compound, drug |
SKGM-2 medium |
Lonza, Cat. CC-3245 |
|
|
| Chemical compound, drug |
ROCK inhibitor Y27632 |
Tocris, Cat. 1254 |
|
0.1% |
| Chemical compound, drug |
Matrigel |
Corning, Cat. 354277 |
|
1 in 40 |
| Chemical compound, drug |
DMEM/F12 medium |
Thermo Fisher Scientific, Cat. 11320033 |
|
|
| Chemical compound, drug |
Knock-out Serum Replacement |
Invitrogen, Cat. 10828028 |
|
2% |
| Chemical compound, drug |
ITS |
Life Technologies, 41400045 |
|
1% |
| Chemical compound, drug |
Pen/Strep |
Life Technologies, Cat. 15140122 |
|
0.2% |
| Chemical compound, drug |
Chiron |
Tocris Bioscience, Cat. 4423 |
|
1 mM |
| Chemical compound, drug |
TGF-β inhibitor SB431542 |
Tocris, Cat. 1614 |
|
10 mM |
| Chemical compound, drug |
Prednisolone |
Sigma-Aldrich, Cat. P6004 |
|
10 mM |
| Chemical compound, drug |
Gibco Fetal Bovine Serum |
Thermo Fisher Scientific, Cat. A4766801 |
|
|
| Chemical compound, drug |
Rhodamine-phalloidin |
Invitrogen, Cat. R415 |
|
1 in 500 |
| Chemical compound, drug |
Alexa568-phalloidin |
Invitrogen, Cat. A12380 |
|
1 in 500 |
| Chemical compound, drug |
Prolong Gold Antifade without DAPI |
Invitrogen, Cat. P36934
|
|
|
| Chemical compound, drug |
Prolong Gold Antifade with DAPI |
Invitrogen, Cat. P36935
|
|
|
| Software, algorithm |
CRISPOR tool |
http://crispor.tefor.net
|
|
|
| Software, algorithm |
CENTURI_Mao_FDomMuscle |
https://github.com/BDehapiot/CENTURI_Mao_FDomMuscle; (Dehapiot, 2022) |
|
|
