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. 2022 Aug 8;2022:4776243. doi: 10.1155/2022/4776243

Figure 1.

Figure 1

Melatonin failed to alleviate the HR-induced ferroptosis in NRF2-silenced MTEC. (a) Real-time PCR and (b) western blot analysis of the expression of NRF2 in MTEC after transfection with control siRNA or three alternative NRF2 siRNAs (NRF2 siRNAs 1, 2, and 3), respectively. (c) Western blotting showing the expression of Slc7a11 and GPX4 proteins in control-siRNA and NRF2-siRNA cells treated with HR and 1 mM melatonin. (d–f) The levels of SOD, GSH-Px, and MDA in control-siRNA and NRF2-siRNA cells treated with HR and 1 mM melatonin were measured. Data represent the mean ± SEM of three to four independent experiments. ∗p < 0.05,  ∗∗p < 0.01, and∗∗∗p < 0.001 compared with the NC group. #p < 0.05,  ##p < 0.01, and###p < 0.001 compared with the HR-treated group.