Figure 10.

Melatonin inhibited FA-induced ferroptosis in mice. (a) Quantification of Acsl4, Cox-2, and GPX4 mRNA levels in kidney tissue by real-time PCR; the results showed that treatment of melatonin or Fer-1 inhibited FA-induced upregulation of Acsl4 and Cox2 mRNA levels and restored FA-induced downregulation of GPX4 mRNA levels. (b–e) Quantitative data analysis of western blots of NRF2, Slc7a11, and GPX4 protein expression; the results showed that treatment of melatonin or Fer-1 inhibited FA-induced upregulation of Slc7a11 protein and restored FA-induced downregulation of NRF2 and GPX4 proteins. Thank you for your revision (f–i) Levels of SOD, GSH-Px, MDA, and Fe²⁺ were measured in the kidney tissue of FA-AKI mice; the results showed MT or Fer-1 reduced the accumulation of lipid peroxidation in kidney after folic acid injury. Data represent the mean ± SEM for 6 mice in each group. ^∗p < 0.05,  ^∗∗p < 0.01, and^∗∗∗p < 0.001 compared with the NC and NC+MT (20 mg/kg) groups. ^#p < 0.05,  ^##p < 0.01, and^###p < 0.001 compared with the FA-treated group.