Figure 3.

Melatonin treatment attenuates the IR-induced renal inflammatory response. (a–c) Melatonin alleviated neutrophil and macrophages recruitment, and then secretion of TNF-α in kidney sections labeled with antibodies against Ly6G, CD68, and TNF-α and score. (d) The mRNA expression levels of IL-1β, TNF-α, MCP-1, and IL-6 in the kidneys was evaluated. Data are presented as the mean ± SEM for six mice in each group. ^∗p < 0.05,  ^∗∗p < 0.01, and^∗∗∗p < 0.001 compared to the Sham and Sham+MT (20 mg/kg) groups. ^#p < 0.05,  ^##p < 0.01, and^###p < 0.001 compared to the IR-treated group. Scale bar = 50 μm.