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. 2022 Aug 8;2022:4776243. doi: 10.1155/2022/4776243

Figure 4.

Figure 4

Melatonin improves mitochondrial function and inhibits ferroptosis in the IR-induced acute kidney injury. (a) Renal tissues were imaged using transmission electron microscopy (TEM). Red arrows indicate mitochondrial cristae disappearance and outer membrane rupture. Scale bar = 200 nm. (b) Western blot analysis of mitochondrial proteins and their relative protein levels of UCP2 (c) and MFN2 (d). (e) Renal relative PGC-1a and TFAM levels were evaluated by RT-PCR. (f) Quantification of mRNA levels of Acsl4, Cox-2, and GPX4 kidney tissue by real-time PCR. (g) Western blots of NRF2, Slc7a11, and GPX4 proteins and their relative protein levels of NRF2 (h), Slc7a11 (i), and GPX4 (j). Data represent the mean ± SEM of six mice in each group. ∗p < 0.05,  ∗∗p < 0.01, and∗∗∗p < 0.001 compared to the Sham and Sham+MT (20 mg/kg) groups. #p < 0.05,  ##p < 0.01, and###p < 0.001 compared to the IR-treated group.