Figure 5.

Melatonin reduces IR-induced ferroptosis in the AKI mouse model. (a) The fluorescent probe DHE (red) indicating ROS in kidneys sections from IR-induced mice. (b, e) Lipid peroxidation levels were measured by 4-HNE and MDA in kidney tissue. (c, d) SOD and GSH-Px levels in kidney tissue after IR-AKI. (f) Fe²⁺ concentration in kidney tissue after IR-AKI. Data are presented as the mean ± SEM for six mice in each group. ^∗p < 0.05,  ^∗∗p < 0.01, and^∗∗∗p < 0.001 compared to the Sham and Sham+MT (20 mg/g) groups. ^#p < 0.05,  ^##p < 0.01, and^###p < 0.001 compared to the IR-treated group. Scale bar = 50 μm.