Figure 7.

Melatonin inhibits HR- or Era-induced ferroptosis in vitro. (a) Quantification of Acsl4, Cox-2, and GPX4 mRNA levels by real-time PCR in the HR group. (b–e) Western blotting of NRF2, Slc7a11, and GPX4 in HR-treated MTEC. (f–i) The levels of SOD, GSH-Px, MDA, and Fe²⁺ were measured in HR-treated MTEC, respectively. (j–l) Quantitative data analysis of western blots for Slc7a11 and GPX4 in the Era-treated group. (m–o) The levels of SOD, GSH-Px, and MDA were measured in Era-treated MTEC. Data represent the mean ± SEM of 3–4 independent experiments. ^∗p < 0.05,  ^∗∗p < 0.01, and^∗∗∗p < 0.001 compared with the NC and NC+MT (1 mM) groups. ^#p < 0.05,  ^##p < 0.01, and^###p < 0.001 compared with the HR- or Era-treated group.