Figure 4.
Effects of CUR intervention on SOX2, ABCG2, and β-catenin expression and β-catenin promoter activity in HCC827 cell spheres. (a) Western blot was used to detect the protein levels of SOX2, ABCG2, and β-catenin in HCC827 cell spheres treated with 200 μM CUR for 48 h. (b) Changes of β-catenin promoter activity in HCC827 cell spheres treated with 200 μM CUR for 48 h were detected by luciferase reporter gene. ACompared with the control group, P < 0.05. (c) qPCR was used to detect the mRNA levels of SOX2, ABCG2, and β-catenin in HCC827 cell spheres treated with 200 μM CUR for 48 h. ACompared with the control group, P < 0.05.