TABLE 1.
Relative intensities of 13C multiplet components and derived relative abundances of intact C2 and C3 fragments in the amino acids
Observed carbon position | Preparationc | Relative intensity of multiplet componenta
|
Relative abundance of intact carbon fragmentb
|
|||||||
---|---|---|---|---|---|---|---|---|---|---|
Is | Id | Id* | Idd | It | f(1) | f(2) | f(2*) | f(3) | ||
Terminal carbons | ||||||||||
Ala-β | ME | 0.12 | 0.88 | 0.05 | 0.95 | |||||
ES | 0.11 | 0.89 | 0.05 | 0.95 | ||||||
Arg-δ | ME | 0.11 | 0.89 | 0.04 | 0.96 | |||||
ES | 0.11 | 0.89 | 0.05 | 0.95 | ||||||
Gly-α | ME | 0.09 | 0.91 | 0.02 | 0.98 | |||||
ES | 0.09 | 0.91 | 0.02 | 0.98 | ||||||
His-δ2 | ME | 0.08 | 0.92 | 0.00 | 1.00 | |||||
ES | 0.09 | 0.91 | 0.03 | 0.97 | ||||||
Ile-γ2 | ME | 0.13 | 0.87 | 0.07 | 0.93 | |||||
ES | 0.11 | 0.89 | 0.04 | 0.96 | ||||||
Ile-δ | ME | 0.32 | 0.68 | 0.31 | 0.69 | |||||
ES | 0.33 | 0.67 | 0.32 | 0.68 | ||||||
Leu-δ1 | ME | 0.15 | 0.85 | 0.09 | 0.91 | |||||
ES | 0.11 | 0.89 | 0.04 | 0.96 | ||||||
Leu-δ2 | ME | 0.87 | 0.13 | 1.00 | 0.00 | |||||
ES | 0.87 | 0.13 | 0.99 | 0.01 | ||||||
Lys-ɛ | ME | 0.11 | 0.89 | 0.04 | 0.96 | |||||
ES | 0.11 | 0.89 | 0.05 | 0.95 | ||||||
Pro-δ | ME | 0.11 | 0.89 | 0.04 | 0.96 | |||||
ES | 0.11 | 0.89 | 0.04 | 0.96 | ||||||
Ser-β | ME | 0.51 | 0.49 | 0.55 | 0.45 | |||||
ES | 0.54 | 0.46 | 0.58 | 0.42 | ||||||
Thr-γ2 | ME | 0.48 | 0.52 | 0.51 | 0.49 | |||||
ES | 0.50 | 0.50 | 0.53 | 0.47 | ||||||
Val-γ1 | ME | 0.11 | 0.89 | 0.05 | 0.95 | |||||
ES | 0.11 | 0.89 | 0.04 | 0.96 | ||||||
Val-γ2 | ME | 0.83 | 0.17 | 0.95 | 0.05 | |||||
ES | 0.87 | 0.13 | 0.99 | 0.01 | ||||||
Central carbons in C3 fragments (different scalar couplings) | ||||||||||
Ala-α | ME | 0.07 | 0.01 | 0.03 | 0.88 | 0.00 | 0.01 | 0.04 | 0.95 | |
ES | 0.08 | 0.01 | 0.03 | 0.88 | 0.00 | 0.01 | 0.05 | 0.94 | ||
Asp-α | ME | 0.18 | 0.09 | 0.32 | 0.41 | 0.16 | 0.09 | 0.37 | 0.38 | |
ES | 0.19 | 0.09 | 0.33 | 0.39 | 0.18 | 0.08 | 0.38 | 0.36 | ||
Asp-β | ME | 0.18 | 0.34 | 0.31 | 0.17 | 0.16 | 0.39 | 0.36 | 0.09 | |
ES | 0.19 | 0.32 | 0.32 | 0.17 | 0.18 | 0.37 | 0.37 | 0.08 | ||
Glu-α | ME | 0.18 | 0.34 | 0.31 | 0.17 | 0.16 | 0.40 | 0.36 | 0.08 | |
ES | 0.19 | 0.33 | 0.32 | 0.16 | 0.18 | 0.39 | 0.38 | 0.07 | ||
Glu-γ | ME | 0.09 | 0.02 | 0.77 | 0.12 | 0.04 | 0.00 | 0.96 | 0.00 | |
ES | 0.09 | 0.02 | 0.77 | 0.12 | 0.03 | 0.01 | 0.95 | 0.01 | ||
His-α | ME | 0.08 | 0.01 | 0.00 | 0.91 | 0.01 | 0.01 | 0.00 | 0.98 | |
ES | 0.08 | 0.00 | 0.00 | 0.92 | 0.00 | 0.00 | 0.00 | 1.00 | ||
His-β | ME | 0.07 | 0.80 | 0.01 | 0.12 | 0.01 | 0.99 | 0.00 | 0.00 | |
ES | 0.08 | 0.79 | 0.01 | 0.12 | 0.02 | 0.98 | 0.00 | 0.00 | ||
Ile-α | ME | 0.18 | 0.03 | 0.70 | 0.10 | 0.16 | 0.00 | 0.84 | 0.00 | |
ES | 0.18 | 0.03 | 0.69 | 0.10 | 0.17 | 0.00 | 0.83 | 0.00 | ||
Leu-α | ME | 0.09 | 0.02 | 0.77 | 0.12 | 0.04 | 0.01 | 0.95 | 0.00 | |
ES | 0.09 | 0.03 | 0.77 | 0.11 | 0.03 | 0.02 | 0.95 | 0.00 | ||
Met-α | MEd | |||||||||
ES | 0.17 | 0.09 | 0.31 | 0.43 | 0.14 | 0.09 | 0.36 | 0.41 | ||
Phe-α | ME | 0.08 | 0.04 | 0.00 | 0.88 | 0.00 | 0.05 | 0.00 | 0.95 | |
ES | 0.08 | 0.01 | 0.00 | 0.91 | 0.01 | 0.01 | 0.00 | 0.98 | ||
Phe-β | ME | 0.07 | 0.81 | 0.01 | 0.12 | 0.00 | 1.00 | 0.00 | 0.00 | |
ES | 0.07 | 0.79 | 0.02 | 0.12 | 0.01 | 0.98 | 0.01 | 0.00 | ||
Pro-α | ME | 0.17 | 0.36 | 0.33 | 0.14 | 0.15 | 0.42 | 0.38 | 0.05 | |
ES | 0.17 | 0.35 | 0.31 | 0.17 | 0.15 | 0.41 | 0.36 | 0.08 | ||
Ser-α | ME | 0.08 | 0.00 | 0.43 | 0.49 | 0.01 | 0.00 | 0.53 | 0.46 | |
ES | 0.08 | 0.01 | 0.45 | 0.46 | 0.01 | 0.00 | 0.56 | 0.43 | ||
Thr-α | ME | 0.17 | 0.10 | 0.32 | 0.41 | 0.15 | 0.10 | 0.37 | 0.38 | |
ES | 0.18 | 0.10 | 0.32 | 0.40 | 0.16 | 0.10 | 0.37 | 0.37 | ||
Tyr-α | ME | 0.07 | 0.01 | 0.00 | 0.92 | 0.00 | 0.01 | 0.00 | 0.99 | |
ES | 0.08 | 0.00 | 0.00 | 0.92 | 0.01 | 0.00 | 0.00 | 0.99 | ||
Tyr-β | ME | 0.07 | 0.81 | 0.01 | 0.12 | 0.00 | 1.00 | 0.00 | 0.00 | |
ES | 0.07 | 0.80 | 0.01 | 0.12 | 0.01 | 0.99 | 0.00 | 0.00 | ||
Val-α | ME | 0.08 | 0.01 | 0.79 | 0.12 | 0.02 | 0.00 | 0.98 | 0.00 | |
ES | 0.08 | 0.03 | 0.77 | 0.12 | 0.02 | 0.03 | 0.95 | 0.00 | ||
Central carbons in C3 fragments (equal scalar couplings) | ||||||||||
Arg-β | ME | 0.41 | 0.52 | 0.07 | 0.50 | 0.50 | 0.00 | |||
ES | 0.42 | 0.51 | 0.07 | 0.51 | 0.49 | 0.00 | ||||
Glu-β | ME | 0.42 | 0.51 | 0.07 | 0.51 | 0.49 | 0.00 | |||
ES | 0.44 | 0.50 | 0.06 | 0.54 | 0.46 | 0.00 | ||||
Ile-γ1 | ME | 0.30 | 0.60 | 0.10 | 0.33 | 0.65 | 0.02 | |||
ES | 0.32 | 0.60 | 0.08 | 0.36 | 0.64 | 0.00 | ||||
Leu-β | ME | 0.75 | 0.22 | 0.03 | 0.99 | 0.00 | 0.01 | |||
ES | 0.76 | 0.22 | 0.02 | 1.00 | 0.00 | 0.00 | ||||
Lys-β | ME | 0.17 | 0.67 | 0.16 | 0.15 | 0.78 | 0.07 | |||
ES | 0.19 | 0.67 | 0.14 | 0.18 | 0.77 | 0.05 | ||||
Lys-γ | ME | 0.44 | 0.50 | 0.06 | 0.54 | 0.46 | 0.00 | |||
ES | 0.43 | 0.51 | 0.06 | 0.53 | 0.47 | 0.00 | ||||
Lys-δ | ME | 0.08 | 0.81 | 0.12 | 0.02 | 0.98 | 0.00 | |||
ES | 0.08 | 0.81 | 0.12 | 0.02 | 0.98 | 0.00 | ||||
Pro-β | ME | 0.40 | 0.53 | 0.07 | 0.48 | 0.52 | 0.00 | |||
ES | 0.41 | 0.52 | 0.07 | 0.50 | 0.50 | 0.00 | ||||
Pro-γ | ME | 0.07 | 0.77 | 0.16 | 0.01 | 0.94 | 0.05 | |||
ES | 0.08 | 0.77 | 0.15 | 0.02 | 0.94 | 0.04 | ||||
Thr-β | ME | 0.19 | 0.67 | 0.14 | 0.18 | 0.77 | 0.05 | |||
ES | 0.19 | 0.65 | 0.16 | 0.18 | 0.75 | 0.07 | ||||
Tyr-δx | ME | 0.14 | 0.74 | 0.12 | 0.11 | 0.88 | 0.01 | |||
ES | 0.15 | 0.74 | 0.11 | 0.12 | 0.87 | 0.01 | ||||
Tyr-ɛx | ME | 0.28 | 0.25 | 0.47 | 0.30 | 0.22 | 0.48 | |||
ES | 0.29 | 0.24 | 0.47 | 0.31 | 0.21 | 0.48 |
Is singlet; Id, doublet split by the smaller coupling; Id*, doublet split by the larger coupling; Idd, doublet of doublets; It, triplet.
Experimental error, ±0.02.
ME, sample harvested in the mid-exponential phase; ES, sample harvested in the early stationary phase.
Not evaluated due to an insufficient signal-to-noise ratio.