TABLE 3.
Exploration of lysine biosynthesis
| Pathway | Carbon atom analyzed | Relative abundance of indicated intact carbon fragmenta at:
|
|||||
|---|---|---|---|---|---|---|---|
| Mid-exponential phase
|
Early stationary phase
|
||||||
| f(1) | f(2) | f(3) | f(1) | f(2) | f(3) | ||
| Experimental | Lys-ɛb | 0.04 | 0.96 | 0.05 | 0.95 | ||
| Lys-β | 0.15 | 0.78 | 0.07 | 0.18 | 0.77 | 0.05 | |
| Diaminopimelate-dehydrogenase variant of the diaminopimelate pathwayc | Lys-ɛ | 0.05 | 0.95 | 0.05 | 0.95 | ||
| Lys-β | 0.16 | 0.75 | 0.09 | 0.18 | 0.74 | 0.08 | |
| Acetylase/succinylase variant of the diaminopimelate pathwayd | Lys-ɛ | 0.29 | 0.71 | 0.30 | 0.70 | ||
| Lys-β | 0.11 | 0.85 | 0.04 | 0.12 | 0.84 | 0.04 | |
| α-Aminoadipatee | Lys-ɛ | 0.04 | 0.96 | 0.04 | 0.96 | ||
| Lys-β | 0.52 | 0.48 | 0.00 | 0.55 | 0.45 | 0.00 | |
a
For the definitions of f(1), f(2), and f(3), see Materials and Methods. All values except the experimental values are expected.
b
Values coincide with those observed for Ala-β.
c
Calculated with equations 7 and 8.
d
Calculated with equations 9 and 10.
e
Calculated with equations 11 and 12.