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(A) SE-HPLC analysis of purified hexS-protein (bottom, black trace) superimposed with a thyroglobulin reference (bottom, dashed line). MW standards were used to estimate hexS-protein size (top trace). (B) RP-HPLC chromatograms showing the performance of different columns on comS-protein A under low TFA conditions. The solvent gradient used in development for all columns is shown by the dashed line.
