Table 2.

Gradient I conditions used in RP-HPLC method.

Time(min)	Flow rate(mL/min)	%A	%B
0	1.0	95	5
2	1.0	95	5
4	1.0	65	35
34	1.0	35	65
37	1.0	5	95
39	1.0	5	95
40	1.0	95	5
45	1.0	95	5