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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Allergy Clin Immunol. 2022 Mar 12;150(2):425–439.e3. doi: 10.1016/j.jaci.2022.02.024

Figure 5.

Figure 5.

The mRNA transcript for T-lymphocyte-associated protein 4 (Ctla4) and transcripts of other Treg cell-related genes are enriched in CD4+ T cells from mLNs of peanut-butter fed mice. (A) Schematic overview of the experimental protocol. (B) Unsupervised t-distributed stochastic neighbor embedding (t-SNE) plots of CD4+ T cells are shown. (C) t-SNE plots of selected genes are shown. (D) Foxp3eGFP mice were treated as described in (A), and mRNA transcript for Ctla4 in sorted Foxp3− and Foxp3+ cells was analyzed by qRT-PCR. Data are presented as the mean ± SEM in each group. *P<0.05 between the groups indicated by a horizonal line.