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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Allergy Clin Immunol. 2022 Mar 15;150(2):373–384. doi: 10.1016/j.jaci.2022.03.002

Table 2.

scRNAseq findings in mouse allergic skin inflammation shared with AD lesional skin

scRNAseq of EC sensitized mouse skin AD Reference
scRNAseq Other
Increased T cell cluster IHC 68
Increased DC cluster IF 69
Increased macrophages cluster X IHC 36,37,62
Increased mast cell/basophils cluster FACS, IF, Histology 70,71
Increased neutrophil cluster IHC 72
Upregulation of Il33 in collagen I-producing fibroblasts IHC 73
Upregulation of Ccl2 in fibroblasts X 36
Upregulation of Postn in fibroblasts X 36
Upregulation of Ccl2 in fibroblasts X 36
Upregulation of Ccl27 in KC basal layer cluster X 36
Downregulation of Krt1 and Krt10 in KC clusters IF 74
Downregulation of Flg in KC basal layer cluster PCR, IHC 75
Downregulation of Lor in KC basal layer cluster PCR, IHC 50
Upregulation of Selp expression in endothelial cell clusters IHC 76,77
Upregulation of Cd4 in T cell cluster IHC 68,78
Upregulation of Ctla4 andTnfrs25 in T cell cluster X IHC 36,37, 79, 80
Upregulation of Il13 in T cell cluster X FACS 36,37,57
Upregulation of Tnfrs4 in T cell cluster X 36, 37
Upregulation of Ahr expression in T cell cluster X FACS 36, 37, 66
Upregulation of Fab5, Ccl8 and ccl6 in macrophage cluster IHC 59
Upregulation of antigen presentation genes in macrophage cluster IHC 62

X: scRNAseq data, IHC: Immunohistochemistry, IF: Immunofluorescence, FACS: Fluorescence-Activated Cell Sorting, PCR: Polymerase Chain Reaction.