Table 2.
scRNAseq findings in mouse allergic skin inflammation shared with AD lesional skin
| scRNAseq of EC sensitized mouse skin | AD | Reference | |
|---|---|---|---|
| scRNAseq | Other | ||
| Increased T cell cluster | IHC | 68 | |
| Increased DC cluster | IF | 69 | |
| Increased macrophages cluster | X | IHC | 36,37,62 |
| Increased mast cell/basophils cluster | FACS, IF, Histology | 70,71 | |
| Increased neutrophil cluster | IHC | 72 | |
| Upregulation of Il33 in collagen I-producing fibroblasts | IHC | 73 | |
| Upregulation of Ccl2 in fibroblasts | X | 36 | |
| Upregulation of Postn in fibroblasts | X | 36 | |
| Upregulation of Ccl2 in fibroblasts | X | 36 | |
| Upregulation of Ccl27 in KC basal layer cluster | X | 36 | |
| Downregulation of Krt1 and Krt10 in KC clusters | IF | 74 | |
| Downregulation of Flg in KC basal layer cluster | PCR, IHC | 75 | |
| Downregulation of Lor in KC basal layer cluster | PCR, IHC | 50 | |
| Upregulation of Selp expression in endothelial cell clusters | IHC | 76,77 | |
| Upregulation of Cd4 in T cell cluster | IHC | 68,78 | |
| Upregulation of Ctla4 andTnfrs25 in T cell cluster | X | IHC | 36,37, 79, 80 |
| Upregulation of Il13 in T cell cluster | X | FACS | 36,37,57 |
| Upregulation of Tnfrs4 in T cell cluster | X | 36, 37 | |
| Upregulation of Ahr expression in T cell cluster | X | FACS | 36, 37, 66 |
| Upregulation of Fab5, Ccl8 and ccl6 in macrophage cluster | IHC | 59 | |
| Upregulation of antigen presentation genes in macrophage cluster | IHC | 62 | |
X: scRNAseq data, IHC: Immunohistochemistry, IF: Immunofluorescence, FACS: Fluorescence-Activated Cell Sorting, PCR: Polymerase Chain Reaction.