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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Mol Neurobiol. 2022 Jun 7;59(8):5104–5120. doi: 10.1007/s12035-022-02905-4

Figure 7. lnc RNA, HOTTIP regulates dendritic polarity of cortical pyramidal neurons.

Figure 7.

(A) Knockdown of HOTTIP inhibits primary apical dendrite development in pyramidal neurons. E14.5 embryos were electroporated in utero with a control or shHOTTIP construct to target cortical pyramidal neurons. The electroporated brains were collected at age P14 and dendrite morphologies in pyramidal neurons expressing GFP were visualized. Scale bar, 25μm. (B) Representative morphologies of control or shHOTTIP-expressing pyramidal neurons with their dendrite formation. (C) The numbers and lengths of dendrites were quantified. n=5 mice for each condition. Statistical significance was determined by two-tailed Student’s t-test. **p < 0.01, ***p < 0.001. (D) Knockdown of HOTTIP shows abnormal orientation of apical dendrites in pyramidal neurons. The numbers on the outer circle indicate the degrees of angles from the right horizontal line. The numbers inside of the outer circle show the lengths of dendrites. n=50 cells from 5 mice for each condition. Statistical significance was determined by two-tailed Student’s t-test. ***p < 0.001.