TABLE 5.

Extracellular pyruvate levels and intracellular metabolite in continuous steady-state cultures of C. cellulolyticum

D (h−1)	Extracellular pyruvate (μM)a	Intracellular metabolite (μmol [g of cells]−1)a
		DHAP	GAP	FBP
0.033	NDb	7.9 ± 0.9	3.8 ± 0.7	1.0 ± 0.1
0.053	ND	8.9 ± 1.0	7.4 ± 1.4	2.1 ± 0.2
0.062	79.0 ± 4.3	15.5 ± 1.6	7.1 ± 1.2	2.1 ± 0.2
0.085	138.7 ± 7.7	21.9 ± 2.3	6.0 ± 1.0	3.4 ± 0.5
0.120	147.3 ± 8.0	30.2 ± 2.9	6.4 ± 1.2	2.4 ± 0.3
0.138	70.2 ± 3.5	32.7 ± 3.2	7.4 ± 1.3	3.2 ± 0.4

^aValues are the averages of three determinations (± standard deviations). 

^bND, not detectable.