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. Author manuscript; available in PMC: 2023 Aug 11.
Published in final edited form as: J Med Chem. 2022 Jul 27;65(15):10611–10625. doi: 10.1021/acs.jmedchem.2c00807

Figure 8.

Figure 8.

Compound 9 reduces the NSD2 and IKZF3 protein levels in 293FT cells in a CRBN-dependent manner. The NSD2 and IKZF3 protein levels were reduced in WT 293FT cells treated with compound 9 for 48 h in a concentration-dependent manner (left), while the NSD2 and IKZF3 protein levels were not affected by the treatment of compound 9 for 48 h in 293FT cells with the CRISPR-Cas9-mediated KO of CRBN (right). The NSD2, IKZF3, and CRBN protein levels were detected by Western blotting with GAPDH as the loading control. The WB results are representative of at least two independent experiments.