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. Author manuscript; available in PMC: 2023 Aug 30.
Published in final edited form as: J Mol Biol. 2022 Jun 17;434(16):167685. doi: 10.1016/j.jmb.2022.167685

Figure 7. TRF2-DNA phase diagram and the implications for multi- and single-DNA condensation.

Figure 7.

A-B. Reconstruction of the phase diagram for mixtures of TRF2 and (T2AG3)2 (A) and TRF2 and (T2AG3)19 (B) in gray as obtained by measuring the intensity of labeled protein and DNA in the dilute and dense phases (full circles) after mixing of the indicated concentrations (crosses). Solid lines connecting the dilute and dense phase concentrations represent expected tie lines across the solution, whereas the dashed lines are guide-to-the-eyes phase boundaries based on the concentration limits determined via fluorescence intensities and turbidity measurements. Addition of Rap1 (magenta area) restricts the phase boundaries. C. Lattice-based multi-component simulations reproduce the observed change in boundaries when increasing length of the DNA or decreasing multivalence of the TRF2 through a mean-field diminution in attractive interactions, mimicking the interpreted effect of hRap1. Only the lower-left portion of the phase diagram in panel B is simulated in panel C (dashed line with yellow highlight).