FIGURE 8.

Digoxin treatment did not alter astrocyte number or function in vitro. After 1 week in culture, mixed male and female rat astrocytes were treated with digoxin (0.1, or 1.0, or 10.0 ng/ml) for 24 h (d1), 48 h (d2), and 96 h (d4). Total RNA was then extracted and microfluidic qPCR used to determine changes in astrocyte functional state. The heatmap shows relative expression of reactive astrocyte transcripts in digoxin‐treated astrocytes compared to vehicle (a). Live phase contrast imaging of astrocytes was carried out at 20× with results presented as cell number per field (ROI) and average number of astrocyte primary processes per cell within each treatment group (b‐d). (ns, not significant). Extended data for Figure 8 is labeled as Figure S4.