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. Author manuscript; available in PMC: 2023 Aug 10.
Published in final edited form as: Cell Host Microbe. 2022 Jul 7;30(8):1163–1172.e6. doi: 10.1016/j.chom.2022.06.006

Figure 4. IsdB vaccination recalls the non-protective IsdB-specific response from prior S. aureus infection.

Figure 4

(A) Alluvial plot showing the top 40 of clonotypes from LAC, LAC/IsdB and N/IsdB.

(B) IsdB-specific clonotype network. Each dot represents cells with identical CDR3 sequence. For each clonotype, the numeric clonotype ID is shown in the graph. The size of each dot refers to the number of cells with the same CDR3.

(C) Rapid rise of specific IgG serum titer 7 days after first IsdB vaccination in SA pre-infected mice (n=10 per mouse group).

(D) ELISpot assay showing IsdB-specific IgG-secreting cells (ASCs) per 5×104 spleen B cells isolated 7 Days after the first IsdB vaccination.

Error bars represent means ± SD. n.s., not significant, *P<0.05, **P<0.01, and ***P< 0.001; one-way ANOVA (C and D). See also Figure S4.