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Analysis of the purified EngE by SDS-PAGE (A) and Western blotting (immunoblotting) (B). Lane M, standard markers (myosin [212 kDa], α2-macroglobulin [170 kDa], β-galactosidase [116 kDa], transferrin [76 kDa], and glutamic dehydrogenase [53 kDa]); lane 1, purified EngE; lane 2, cellulosomal proteins from C. cellulovorans.
