TABLE 2.
TLC analysis of hydrolysis products
| Enzyme | Product(s) formed with the following
substratea:
|
Reference | ||
|---|---|---|---|---|
| 3G | 4G | 5G | ||
| EngB | (2G) | 2G, 3G | 2G, 3G | 14 |
| EngD | (1G), (2G) | (1G), 2G, 3G | (1G), 2G, 3G | 14 |
| EngF | None | (2G), (3G) | 2G, 3G, 4G | 14 |
| EngEb | 2G | 2G | 2G | This study |
a
Products in parentheses showed faint but significant spots on TLC plates.
b
The reaction mixture contained 1.34 μg of purified EngE and 3G, 4G, and 5G in 50 mM sodium acetate buffer (pH 5.0). The reaction was performed at 37°C for 16 h.