Fig. 5. Influence of inhibitors on 2-HG levels in cells bearing recombinant R132C, R132C/S280F, R132H and R132H/S280F.

LN18 cells were treated with the inhibitors ivosidenib, GSK864, FT-2102, IDH224, and DS-1001B in DMSO (final concentration: 5 µM). 2-HG levels were determined by anion-exchange chromatography coupled to MS⁵¹ (errors: standard errors of the mean, n = 4 independent replicates). Note that, whilst ivosidenib is not/poorly active in reducing 2-HG levels in R132C/S280F and R132H/S280F bearing cells, the other inhibitors are active in reducing 2-HG levels. Control cells were generated by transduction with lentiviral vectors containing no IDH1. Box-and-whisker plots: The centre line is the median and the bounds are 25th and 75th percentile values. The whiskers are the minimum and maximum measured 2-HG levels for each experimental group. Source data are provided as a Source Data file.