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. 2022 Aug 15;13(8):707. doi: 10.1038/s41419-022-05101-3

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© The Author(s) 2022, corrected publication 2022

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Fig. 1 — a GFP-IRF-3 HeLa cells were injected with poly I:C (1 μg/μl) and observed live for the localization of IRF-3 at the indicated times after the injection. The injection was performed for a colony of cells, each cell was followed for the nuclear translocation of IRF-3, and the % of nuclear IRF-3 was calculated (number of cells with nuclear IRF-3/number of injected cells). b GFP-IRF-3 HeLa cells were injected with the indicated RNA/protein. GG25: 5’-ppp-RNA; poly I:C: long poly I:C; RIG-I: recombinant RIG-I protein; ΔTM-IPS-1: recombinant IPS-1 protein devoid of the transmembrane domain. The amount of injected RNA or protein (μg/μl) is indicated at the bottom of the graph. IFN-β priming indicates the pretreatment of cells with IFN-β (1000 U/ml for 12 h) prior to the injection. At 3 h, injected cells were observed live for the localization of IRF-3, and % nuclear IRF-3 was calculated as in (a) and indicated at the top of each bar. c GFP-IRF-3 HeLa cells were injected with the indicated RNA/protein. Cells were fixed after 3 h and observed for IRF-3 (green) or the expression of IFNB mRNA (FISH, red). Scale bar = 25 µm.