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. 2022 Aug 15;13(8):707. doi: 10.1038/s41419-022-05101-3

Fig. 3. Diverse mechanisms of cell death induced by GG25 and poly I:C.

Fig. 3

a GFP-IRF-3 HeLa, GFP-Δ1-58IRF-3 HeLa, and GFP-IRF-3 IFNAR1 KO HeLa cells were injected with RIG-I and GG25 (1 μg/μl each), observed live for cell death, and quantified for % cell survival at the indicated time points. b HeLa cells and indicated KO HeLa cells were injected with poly I:C (1 μg/μl), observed for cell death, and % cell survival was quantified. c GFP-IRF-3 HeLa cells were mock treated, treated with poly I:C (5 μg/ml in culture medium for 3 h), injected with poly I:C (1 μg/μl for 3 h), transfected with poly I:C (0.5 μg/ml in culture medium with lipofectamine for 3 h), injected with RIG-I and GG25 (1 μg/μl each for 3 h), or transfected with GG25 (4 μg/ml in culture medium with lipofectamine for 3 h). Cells were fixed and stained for TIAR and G3BP1 with respective antibodies for microscopy. Scale bar = 25 µm.