| Antibodies |
| Mouse anti-Lamin B antibody |
DSHB |
ADL67.10; RRID: AB_528336 |
| Mouse anti-Lamin B antibody |
DSHB |
ADL84.12; RRID: AB_528338 |
| Rabbit anti-GFP antibody |
Invitrogen |
A21311; RRID: AB_221477 |
| Rabbit anti-RFP antibody |
MBL International |
PM005, RRID: AB_591279 |
| Rabbit anti-Rh4 antibody |
gift from C. Zuker, Columbia University |
N/A |
| Mouse anti-Rh3 antibody |
gift from S. Britt, University of Texas at Austin |
N/A |
| Rat anti-Elav antibody |
DSHB |
7E8A10; RRID: AB_528218 |
| Guinea pig anti-Ss antibody |
gift from Y.N. Jan, University of California, San Francisco |
N/A |
| Deposited Data |
| Predicted PRE data |
modENCODE |
FlyBase ID FBlc0000414 |
| scATAC-deq data |
UCSC Genome Browser Custom Track |
Available at: http://genome.ucsc.edu/s/cbravo/Bravo_et_al_EyeAntennalDisc
|
| Experimental Models: Organisms/Strains |
|
yw ; + ; Df(3R)Exel6269
|
Bloomington Stock Center |
7736; RRID: BDSC_7736 |
|
yw ; ey>Gal4, UAS>flp/+ ; FRT82B ash21, e, red/GMR>hid
|
Bloomington Stock Center |
5253; RRID: BDSC_5253 |
|
yw ; FRT40A lid140 ; FRT40A GMR>hid ; ey>Gal4, UAS>flp/+
|
Bloomington Stock Center |
76954; RRID: BDSC_76954 |
|
Ato(384)>Gal4/yw ; UAS>klu/+ ; P{y[+t7.7] w[+mC]=20XUAS-6XGFP}attP2/+
|
Bloomington Stock Center |
52262; RRID: BDSC_52262 |
|
yw, M{3xP3-RFP.attP}ZH-2A ; + ; + ; M{RFP[3xP3.PB] GFP[E.3xP3]=vas-int.Dm}ZH-102D
|
Bloomington Stock Center |
24480; RRID: BDSC_24480 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>GFP RNAi/+
|
Bloomington Stock Center |
35786; RRID: BDSC_35786 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>Cp190 RNAi/+
|
Bloomington Stock Center |
35078; RRID: BDSC_35078 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>su(Hw) RNAi/+
|
Bloomington Stock Center |
33906; RRID: BDSC_33906 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>mdg4 RNAi/+
|
Bloomington Stock Center |
33907; RRID: BDSC_33907 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>trx RNAi/+
|
Bloomington Stock Center |
33703; RRID: BDSC_33703 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>ash2 RNAi v20/+
|
Bloomington Stock Center |
64942; RRID: BDSC_64942 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>ash2 RNAi v22/+
|
Bloomington Stock Center |
35388; RRID: BDSC_35388 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>Mnn1 RNAi/+
|
Bloomington Stock Center |
35150; RRID: BDSC_35150 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>Trl RNAi/+
|
Bloomington Stock Center |
67265; RRID: BDSC_67265 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>Pc RNAi/+
|
Bloomington Stock Center |
36070; RRID: BDSC_36070 |
|
elav>Gal4/w ; UAS>Dcr2/+ ; UAS>lid RNAi/+
|
Bloomington Stock Center |
28944; RRID: BDSC_28944 |
| Oligonucleotides |
| Oligos for the ss locus deletion CRISPR |
See Table S4 for homologous bridge sequence, mutation size, and phenotypic effect |
N/A |
| Oligos for the repression reporter insert CRISPR |
See Table S5 for homologous bridge sequence, mutation size, and phenotypic effect |
N/A |
| Software and Algorithms |
| MATLAB_R2019b |
MathWorks |
https://www.mathworks.com
|
| ImageJ |
(Schindelin et al., 2012) |
https://imagej.nih.gov/ij/
|
| Script used to generate 19-bp barcoding primers for Oligopaints probe design |
(Viets et al., 2019) |
Available at: https://github.com/kviets0913/Oligopaints-Primers-Custom-Script
|
| Custom script used to analyze images and quantify the density of nascent RNA spots |
Generated for this study |
Available at: https://github.com/lvoortman/Automated_Image_Analysis
|
