Figure 3.

Involvement of apoptosis and necroptosis mechanisms in Pla2g6^−/− mice treated with semaglutide once weekly. Relative quantification of (A) BClxL, (B) BCl2 and (C) Bax with Gapdh as internal reference by quantitative PCR. RT-qPCR performed from RNA samples prepared from WT, untreated Pla2g6^−/− and treated Pla2g6^−/− (Pla2g6^−/− Sema) brain samples (n = 5 per group). (D) Western blot analysis and quantification of the expression of (E) RIP1 and (F) RIP3 in WT, untreated Pla2g6^−/− semaglutide treated Pla2g6^−/− brain samples at week 14 (n = 3 per group). Protein quantification was normalised with β-actin. All data are presented as mean (SEM); ordinary one-way ANOVA adjusted using Tukey’s multiple comparisons test; p values reported in Table S6. * indicates statistically significant difference between the relevant experimental group and WT controls; # indicates statistically significant difference between Pla2g6^−/− Sema mice and untreated Pla2g6^−/− controls.