Table 1.
Data collection and refinement statistics
| Data collection | DLC1 RhoGAP + p120RasGAP SH3 |
|---|---|
| PDB accession code | 7TPB |
| Wavelength (Å) | 0.97918 |
| Resolution range (Å) | 50–3.20 (3.31–3.20) |
| Space group | H 3 |
| Cell dimensions a, b, c (Å) | 143.7, 143.7, 152.8 |
| α, β, γ (°) | 90, 90, 120 |
| Unique reflections | 19420 |
| Multiplicity | 9.1 (7.8) |
| Completeness (%) | 100 (100) |
| Mean I/σI | 9.9 (1.6) |
| Wilson B factor (Å2) | 80.4 |
| Rpim (%) | 9.0 (65.7) |
| CC½ | 99.3 (33.2) |
| CC∗ | 99.8 (70.6) |
| Refinement | |
| Resolution range (Å) | 48.25–3.20 (3.37–3.20) |
| Reflections used in refinement | 18492 (2658) |
| Reflections used for Rfree | 920 (122) |
| % Reflections used for Rfree | 4.7 (4.6) |
| Rwork (%) | 21.2 (30.1) |
| Rfree (%) | 26.0 (33.2) |
| No. of non-hydrogen atoms | |
| Protein | 8096 |
| RMSD | |
| Bond lengths (Å) | 0.002 |
| Bond angles (°) | 0.474 |
| Ramachandran plot | |
| Favored, allowed, outliers (%) | 99.2, 0.8, 0 |
| Rotamer outliers (%) | 0 |
| MolProbity clashscore | 5.7 (100th percentile) |
| Average B factor (Å2) | |
| Overall | 85.5 |
| Copies A, C, E, G (SH3) | 84.6, 92.8, 101.5, 105.7 |
| Copies B, D, F, H (RhoGAP) | 73.5, 80.6, 83.2, 91.2 |
Statistics for the highest resolution shell are shown in parentheses. RMSD root-mean-square deviation.