Table 2.
IC50 calculation for p120RasGAP inhibition of DLC1 RhoGAP activity
| SH3 (Fig. 4b) | IC50 (μM) | 95% CI (μM) | R2 |
|---|---|---|---|
| WT | 0.63 | 0.48 to 0.83 | 0.8 |
| Y290A | 2.5 | 1.9 to 3.3 | 0.7 |
| E298A | 20 | 12 to 36 | −1.6 |
| SH2-SH3-SH2 (Fig. 4d) | |||
| WT | 2.9 | 2.4 to 3.6 | 0.8 |
| E298A | 115 | 67 to 310 | 0.2 |
| SH3 WT | 1.7 | 1.5 to 2.0 | 0.93 |
Top: p120RasGAP SH3 domain and mutants. The Y290A and E298A mutants have increased IC50 values relative to the wildtype. Negative R2 value for E298A indicates ineffective inhibition. Bottom: p120RasGAP SH2-SH3-SH2 domains compared to SH3 domain. All values are calculated using GraphPad (GraphPad Prism Version 9).