TABLE 2.

List of engineered streptococcal endolysin (chimeolysin), including their origin, host range, enzymatically active domains (EADs), and cell wall-binding domains (CBDs).

Chimeolysin/Artilysin	Lysin origin	Host range	Enzymatically active domain	Cell-wall binding domain	References
B30 fused with lysostaphin	B30 endolysin and Staphylococcus simulans lysostaphin	S. agalactiae, S. aureus, S. uberis, S. dysgalactiae	(i) CHAP endopeptidase, lysozyme (from B30), and glycylglycine endopeptidase (from lysostaphin) (ii) CHAP endopeptidase (from B30) and glycylglycine endopeptidase (from lysostaphin)	(i) SH3b from B30 and SH3b from lysostaphin (ii) SH3b from lysostaphin only	Donovan et al., 2006a
ClyJ, ClyJ-3, ClyJ-3m	PlyC endolysin and gp20 putative lysin	S. pneumoniae	CHAP domain of PlyC and amidase-2 from gp20	Choline-binding domain from gp20	Luo et al., 2020; Yang et al., 2019
ClyR	PlyC endolysin and PlySs2 endolysin	S. pyogenes, S. agalactiae, S. dysgalactiae, S. equi, S. mutans, S. pneumoniae, S. suis and S. uberis, MRSA, VISA	CHAP domain of PlyC	SH3b domain from PlySs2 (PlySb)	Yang et al., 2015
Cpl-7S	Cpl-7 endolysin	S. pneumoniae, S. pyogenes, E. faecalis, and S. mitis	Lysozyme	15 amino acid substitution in CW_7 repeats	Diez-Martinez et al., 2013
Cpl-711	Cpl-1 endolysin and Cpl-7S endolysin	S. pneumoniae	Lysozyme from Cpl-7S	Linker and choline-binding domain from Cpl-1	Diez-Martinez et al., 2015
Csl2	Cpl-7 endolysin and LySMP endolysin	S. suis, S. mitis, S. oralis, S. pseudopneumoniae	Lysozyme from Cpl-7	Cpl-7-like CBD (CW_7 repeats) from LySMP	Vazquez et al., 2017
PL3	Pal endolysin and LytA autolysin	S. pneumoniae (including multi-resistant strains), S. pseudopneumoniae, S. mitis, S. oralis	Amidase from Pal	Choline-binding domain from LytA	Blazquez et al., 2016

CHAP, cysteine, histidine-dependent amidohydrolases/peptidases; MRSA, methicillin-resistant Staphylococcus aureus; VISA, vancomycin intermediate Staphylococcus aureus.