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Effects on Aβ25–35-induced neurite atrophy in primary cortical neurons. Cortical neurons were cultured for 3 days followed treatment with Aβ25–35 for another 3 days, and then treated with drugs (0, 0.1, or 1 μM) 4 days after removing the Aβ-containing medium. The cells were double immunostained with MAP2 and DAPI. The MAP2-possitive neurons were quantified. n = 3. ###p < 0.001 vs. Control, *p < 0.05 vs. Aβ25–35, **p < 0.01, ***p < 0.001. Scale bar = 100 μm.
