Figure 3.

The inhibitory function of quercetin on rotavirus infection is independent of interferon signaling (A) Type I IFN signaling mutant vero cells were infected with RV-SA11 (MOI 3) in the presence of increasing concentrations of quercetin (12.5, 25, and 50 μM) for 12 hpi and cell lysates were subjected to Western blot to evaluate the expression of VP6, whereas β-actin was taken as internal loading control. (B) The activation of JAK1-STAT1 signaling was checked by Western blot performed from MA104 cell lysates mock-infected or RV-SA11-infected and treated with either DMSO or quercetin (50 μM). IFNα/β conjugate (500 units/ml) was treated as positive control. Protein expression and phosphorylation of JAK1 and STAT1 were assessed along with β-actin as internal loading control.