Figure 5.

Quercetin hinders rotavirus infection in mice model. (A,B) Expressions of VP6 and β-actin proteins were assessed by Western blot performed from the small intestinal tissue of 4-day-old suckling mice infected with (A) rotavirus murine strain Ew and (B) rotavirus simian strain SA11 followed by quercetin treatment after 24 and 48 h. (C,D) Stool samples were collected from suckling mice infected with RV and treated either with DMSO or quercetin for 48 h. Total virus particles were quantified afterward. Each bar represented mean ± SD of three independent experiments (Unpaired student's t-test, *p < 0.05, **p < 0.01). (E) HE staining of small intestinal tissue section of 5-day-old mice either uninfected or infected with RV-SA11 in the presence or absence of quercetin (10 mg/kg/day). Scale bars are 10 μm and 30 μm.