TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR (φ80lacZ ΔM15) | Laboratory collection |
| BL21(DE3) | F−ompT hsdSB(rB−mB−) gal dcm (DE3) | Novagen |
| SPC105 | MC4100 (ΔlacU169 araD rpsL relA thi flbB) containing a chromosomal PmarII::lacZ fusion at the λ attachment site and a wild-type mar locus | 7 |
| Plasmids | ||
| pACT7 | Low-copy-number T7 RNA polymerase expression vector | 14 |
| pET11d | High-level expression vector (bla lacI) | Novagen |
| pET13a | Kanamycin-resistant version of pET11a | 22 |
| pAC-MarR (WT) | Low-copy-number wild-type MarR expression vector derived from pACT7 | This study |
| pmarO | pET11d derivative lacking lacI and containing PmarII-marO | This study |
| pSup-Test | pET11d derivative lacking lacI and containing a PmarII-marO::-ccdB fusion | This study |