TABLE 2.

Survival of DH5α containing pSup-Test and a plasmid bearing wild-type MarR or putative superrepressor MarR mutant on gradient plates containing inducing compounds

Inducer	Concn (mM)a
	pET11db	pmarOb	pSup-Testb	Wild-type MarR	MarR mutant
					D26N	D26N/R27H	G95S	V132M	L135F
Plumbagin	>0.4	>0.4	≤0.01	0.01	0.2	0.1	0.1	0.2	0.06
2,4-Dinitrophenol	>0.4	>0.4	0.04	0.05	0.4	0.4	0.4	0.2	0.08
Sodium salicylate	>10	>10	≤0.01	0.5	7.5	6.9	6.7	5.8	2.6
Sodium benzoate	>30	>30	1.4	1.4	18.9	16.7	15.8	17.8	4.4
Menadione sodium metabisulfite	>5	>5	1.6	2.8	3.5	3.6	3.5	3.1	2.7

^aConcentration of the inducer at which growth was inhibited in gradient plates. These values were calculated by measuring the point where growth stopped and dividing it by the total length (90 mm) of the gradient plate. This number was then multiplied by the highest concentration of inducer in the gradient plates, which were as follows: plumbagin, 0.4 mM; 2,4-dinitrophenol, 0.4 mM; sodium salicylate, 10 mM; sodium benzoate, 50 mM; menadione sodium metabisulfite, 5 mM. Results are representative of experiments performed in triplicate. 

^bFor details, see Table 1.