TABLE 3.
Assay of MarR DNA binding activity by SspI restriction enzyme site protection
| Purified MarR | MarR activity (U/mga) | Fold increase in DNA bindingb |
|---|---|---|
| Wild type | 5,495 | 1 |
| D26N mutant | 89,286 | 16.2 |
| G95S mutant | 166,666 | 30.3 |
| L135F mutant | 48,544 | 8.8 |
a
Specific activity was determined based on 50% protection of the SspI recognition enzyme sequence in site I of the mar operator (designated as 5 U of activity). Results are representative of experiments performed at least twice.
b
Relative to DNA binding of the wild-type protein.