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. 2022 Aug 2;13:939127. doi: 10.3389/fimmu.2022.939127

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Copyright © 2022 Chen, Zhang, Zhu, An, Jiang, Yang, Ma, Yang and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Treg (CD3⁺CD4⁺CD25⁺CD127^low/−), metacluster (CD3⁺CD4⁺CD25⁺CD127⁺), and their cytokines expression. (A) Manual gating strategy for metacluster (CD3⁺CD4⁺CD25⁺CD127⁺); (B) the dot plot figures showed the percentage of Treg and the cytokines of the IL-4, IL-10, IL-13, and IFN-γ; (C) the percentage and cytokines (IL-4, IL-10, IL-13, and IFN-γ) of metacluster (CD3⁺CD4⁺CD25⁺CD127⁺) are shown. MC, metacluster (CD3⁺CD4⁺CD25⁺CD127⁺); ABPA, allergic bronchopulmonary aspergillosis; A.f (+) asthma, Aspergillus fumigatus-sensitized asthma; HDM (+) asthma, house dust mite-sensitized asthma; Th1, T helper cells 1; Th2, T helper cells 2; Tfh, follicular helper T cells; Treg, regulatory T cells; IFN-γ, interferon γ; IL-4, interleukin 4; IL-13, interleukin 13; IL-10, interleukin 10. *p<0.05.