Table 1. Linearity, accuracy (%) and precision (%) for the quantification of efavirenz in cervicovaginal swab (CVS).
Nominal
concentration (ng/mL) |
Measured concentration (inter-
day) |
Measured concentration (intra-
day) |
||||||
---|---|---|---|---|---|---|---|---|
mean | SD | precision
(%CV) |
accuracy
(%) |
mean | SD | precision
(%CV) |
accuracy
(%) |
|
25 | 25.7 | 1.31 | 5.10 | 102.7 | 25.6 | 1.63 | 6.36 | 102.3 |
50 | 47.2 | 4.44 | 9.40 | 94.5 | 48.0 | 5.32 | 11.1 | 96.0 |
100 | 91.9 | 6.61 | 7.19 | 91.9 | 91.8 | 4.75 | 5.18 | 91.8 |
200 | 193 | 11.6 | 6.00 | 96.4 | 195 | 17.3 | 8.89 | 97.3 |
500 | 498 | 41.9 | 8.41 | 99.5 | 480 | 57.0 | 11.9 | 96.0 |
1000 | 1010 | 70.9 | 7.00 | 101.4 | 1030 | 93.1 | 8.99 | 103.5 |
2000 | 2030 | 120 | 5.89 | 101.4 | 2005 | 112.3 | 5.60 | 100.3 |
4000 | 3990 | 208 | 5.22 | 99.8 | 4050 | 138.3 | 3.41 | 101.3 |
7500 | 7780 | 402 | 5.17 | 103.7 | 7940 | 457 | 5.75 | 105.9 |
10000 | 10400 | 768 | 7.39 | 104.0 | 10530 | 1020 | 9.68 | 105.3 |
25 (LLOQ) | 24.6 | 2.01 | 8.14 | 98.5 | 24.2 | 1.86 | 7.69 | 96.9 |
75 (LQC) | 75.0 | 6.33 | 8.44 | 99.9 | 74.3 | 4.38 | 5.90 | 99.1 |
4500 (MQC) | 4640 | 457 | 9.85 | 102.7 | 4740 | 496 | 10.5 | 105.3 |
8500 (HQC) | 8440 | 983 | 11.6 | 105.2 | 8470 | 1260 | 14.9 | 99.6 |