Table 3. Matrix effects from different donors in heparin plasma and different anticoagulants.
Donors A to F are individual donors collected using sodium heparin as anticoagulant. Other anticoagulants were collected from individual donors and are not from the same source.
| Concentration | Donor | EDTA | CPD | Li-Hep | Na-Hep haemolysis | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | |||||
| QC 1, 2.97 µg/ml | 1.12 | 1.18 | 1.10 | 1.03 | 1.07 | 1.04 | 1.10 | 1.27 | 1.10 | 1.17 |
| QC 3, 155 µg/ml | 0.93 | 0.87 | 0.93 | 0.91 | 0.93 | 0.88 | 0.93 | 0.96 | 0.90 | 0.91 |
| IS for QC 1, 2 µg/ml | 0.99 | 1.03 | 1.00 | 1.01 | 1.01 | 1.01 | 1.03 | 1.02 | 1.03 | 1.05 |
| IS for QC 3, 2 µg/ml | 1.01 | 1.05 | 1.03 | 1.05 | 1.01 | 1.03 | 1.04 | 1.04 | 1.03 | 1.05 |
| Normalised QC1/IS | 1.13 | 1.15 | 1.10 | 1.02 | 1.06 | 1.02 | 1.06 | 1.24 | 1.07 | 1.12 |
| Normalised QC3/IS | 0.93 | 0.84 | 0.90 | 0.87 | 0.92 | 0.86 | 0.89 | 0.92 | 0.88 | 0.87 |
Hep, Heparin; QC, Quality Control; IS, internal standard.